Figure 2.
Bcl-xL and protease inhibition can block loss of mitochondrial membrane potential. Jurkat T cells were transfected with a bcl-xL expression vector (Bcl-xL) or control vector (Neo) and treated with anti-Fas in the presence and absence of zVAD as described. At the indicated time points, cells were removed for FACS analysis of viability by propidium iodide staining (y axis; PI) and changes in mitochondrial membrane potential by rh123 staining (x axis; Rh123). The data are presented as one of three determinations from a representative experiment. Similar data were obtained in at least five independent experiments performed with three independent clones of each transfectant.