Figure 3.
Bcl-xL-transfected cells can proliferate following removal of anti-Fas. (A) Jurkat T cells transfected with a bcl-xL expression vector (Bcl-xL, □) or a control vector (Neo, ○) were incubated with 100 ng/ml of anti-Fas for a 96-hr time course. At indicated time points, cells were removed for determination of viability by propidium iodide exclusion and for cell counts. The number of viable cells was determined by multiplying the cell count by the fraction of cells that exclude propidium iodide. (B) Jurkat T cells transfected with a bcl-xL expression vector (Bcl-xL, ▪) or a control vector (Neo, •) were treated with 100 ng/ml of anti-Fas for 6 hr. Cells were isolated by centrifugation, washed three times, and resuspended in fresh medium. Cell counts were performed after washing to determine cell loss due to washing and counts adjusted accordingly. Viable cell number was determined as described in A. The data are presented as the mean ± standard deviation of three separate determinations from a representative of two individual experiments.