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. 1997 Apr 15;94(8):3795–3800. doi: 10.1073/pnas.94.8.3795

Figure 2.

Figure 2

Expression of a 284-bp fragment corresponding to murine GLUT3 mRNA in early mouse embryos. Each embryo lane was produced using a cDNA sample derived from RNA of 10 embryos. Brain and 3T3 fibroblast cell lanes were produced using cDNA derived from ≈20 ng RNA. The RNA preparations were reverse transcribed and amplified by 40 cycles of PCR as described. Lanes: L, DNA ladder (bands from top to bottom: 603, 303, 291/281, 234, and 194 bp); 1, negative control (no cDNA); 2, brain; 3, 3T3 fibroblasts; 4, fertilized eggs; 5, two-cell embryos; 6, four-cell embryos; 7, morulae; 8, blastocysts.