Figure 7.

Immunoblot of GLUT3 expression in embryos following antisense oligodeoxynucleotide treatment. Groups of 80 two-cell embryos were cultured over 48 hr in BMOC2 (lane 2) or BMOC2 supplemented with 30 μM of either GLUT3 sense (lane 3) or GLUT3 antisense (lane 4). Resultant embryos were lysed, electrophoresed, and blotted with anti-GLUT3 antibody as described. Rat brain (lane 1), included as a positive control for GLUT3, showed a band at about 55 kDa which was also present in embryos from all treatments. GLUT3 immunoreactivity, however, was markedly reduced in antisense-treated embryos relative to control and sense treatments.