Figure 2.

Nucleotide sequence of an AFGP polyprotein gene and the encoded amino acids from B. saida subclone Bs3–1 aligned with a 5′ AFGP cDNA (A), and D. mawsoni subclone Dm3L (B). Dots represent identical nucleotides between the B. saida AFGP gene and cDNA sequences. The introns are shown in lowercase letters. The distinct spacer sequences linking multiple AFGP sequences are indicated in shaded boxes (Bs3–1) or open boxes (Dm3L). ▾, Putative cleavage site for the signal peptide in both genes. The underlined sequences in Dm3L (B) are regions that share high sequence identities with notothenioid trypsinogen gene (12); not all of the similar intron sequence is shown.