Figure 1.

Binding site selection for mouse Whn DNA binding domain. Double-stranded oligonucleotides randomized at 20 positions were incubated with the bacterially expressed mouse Whn DNA binding domain. After six rounds of affinity purification, bound oligonucleotides were cloned and sequenced. All sequences contained an identical tetranucleotide, 5′-ACGC. The frequency of nucleotides occurring in the flanking regions is indicated by a percentage. No sequence specificity was detected upstream or downstream of the shown 11-bp region.