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. Author manuscript; available in PMC: 2007 Nov 2.
Published in final edited form as: Mol Cell. 2007 Sep 21;27(6):976–991. doi: 10.1016/j.molcel.2007.07.023

Figure 4. Structure of the calponin homology domains of EB1 and Bim1p.

Figure 4

Ribbon diagram of the N-terminal CH domain from EB1 (A) and Bim1p (B) centered on the highly conserved α6 helix. C) Least-squares fit of EB1 (color) and Bim1p (grey) CH domains with cylindrical helix representation showing the overall structural conservation between these two members. D) 2Fo-Fc electron density map at 1.25 Å resolution of the EB1 CH domain structure contoured at 1.0 σ showing the highly conserved aromatic core. E) 90° rotation of the EB1 CH domain about the y-axis shown above in ribbon format for orientation and below in CPK representation for conservation mapping and to summarize mutagenesis results. Center: CH domain residues with 80% identity across species are represented in green, 80% conservation in yellow (D-E)(see Figure S3). Bottom row: results of CH domain mutagenesis on the ability of EB11-187-LZ to plus end track are mapped: ablation of microtubule association: brick red (three cluster mutants indicated: [S16A, R17E, H18E, D19R], [K59E, K60E], [K66E, L67D]); no effect on microtubule plus end tracking: slate.