Figure 3.

Physiological and subphysiological levels of FRQ protein are sufficient to effect repression of frq mRNA levels. (Left) Western blot analysis of FRQ protein induced by incubation of frq⁹qa-2pfrq with QA over time. The number of molecules of FRQ protein per nucleus was calculated relative to a standard curve determined with in vitro translated FRQ (see Materials and Methods). (Dashed box) Analysis of FRQ protein levels seen during the course of a typical circadian cycle in constant conditions (DD 11.5 and 19, respectively, for CT0 and CT8) or in LL in a frq⁺ strain.