Diagram of DNA cloned in the chromosomal walk. The 400-kb chromosomal walk is diagrammed. The position of the 88b.1 cDNA used to initiate the walk is indicated with an arrow. The numbers below the line indicate cytological locations determined by in situ hybridization of probes from the walk to polytene chromosomes. The extent of the chromosomal deficiencies are indicated, as are the approximate locations of the k43, red, trx, and su(Hw) genes. The region of overlap between Df k43γ5 and Df redP52 is approximately 50 kb, and the leftmost 20 kb is presented in greater detail in Fig. 2.