Figure 2.
Restriction map and transcript map of the k43 region. Six ovarian transcripts were identified within the leftmost 16 kb of interval of overlap between Df k43γ5 and Df redP52, as shown. The regions of DNA that hybridize to the various transcripts are summarized as the transcript map, as shown. The four indicated rescue fragments were introduced into the Drosophila genome by P element-mediated transformation. The 10.5-kb BamHI to XbaI fragment and the 7.5-kb HpaI to XbaI fragments, indicated by asterisks, were able to completely rescue the k43 lethal- and female-sterile phenotypes. Multiple independent transformant lines were tested for each construct. As demonstrated by the Northern analysis, only the 2.5-kb transcript is contained entirely within the fragments able to rescue, indicating that this transcript is k43. H, HpaI; R, EcoRI; B, BamHI; A, ApaI; Bg, BglII; Hd, HindIII; X, XbaI.