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Distribution of BPDE adducts along P53 sequences in plasmid DNA differing in methylation status. DNA was methylated (+SAM) or mock-methylated (−SAM) with the CpG-specific methylase SssI, modified with BPDE (0.2 μM), and the distribution of adducts was analyzed by UvrABC incision and LMPCR. (A) Exon 5, nontranscribed strand. (B) Exon 7, nontranscribed strand. (C) Exon 8, nontranscribed strand. Stars mark codons containing CpG dinucleotides.
