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. 1997 Apr 15;94(8):3899–3902. doi: 10.1073/pnas.94.8.3899

Figure 2.

Figure 2

Analysis of ALL1 germ-line and rearranged bands in an AML patient with a normal karyotype (UPN 211) and the partial tandem duplication of ALL1 spanning exons 2–6. (A) Southern blot analysis of HindIII digests of normal control (lane 1) and patient (lane 2) genomic DNA using the B859 probe. Patient genomic DNA was obtained from a specimen containing 99% blasts. Phosphorimage analysis showed the signal intensity of the patient’s germ-line band to be approximately twice that of the rearranged bands (data not shown). (B) Schematic of the genomic structure of the wild-type ALL1 allele showing location of HindIII (H) restriction sites within the gene. Numbered vertical lines and boxes indicate exons. The brackets indicate the 14-kb germ-line (G) ALL1 band detected with the B859 probe after HindIII digestion. (C) Schematic of the genomic structure of the partial tandem duplication of ALL1 involving a portion of the gene spanning exons 2–6, within the white region. The size of the rearranged (R) band that results after a restriction digest of genomic DNA with HindIII and hybridization with the B859 probe is indicated. Note that the chromosome 11 with the partially duplicated ALL1 allele also has a germ-line band after HindIII digestion and hybridization with the B859 probe.