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. 1997 Apr 15;94(8):3926–3931. doi: 10.1073/pnas.94.8.3926

Figure 2.

Figure 2

Effects of decorin or LAP on TGF-β bioactivity in supernatants of PBMCs from TB patients. PBMCs from TB patients and healthy tuberculin-reactive control subjects were cultured in medium alone and medium containing PPD alone or together with decorin (50 μg/ml) or LAP (100 ng/ml). Culture supernatants were obtained after 72 h and added at 50% (vol/vol) to Mv1Lu cells and incubated overnight. [3H]Thymidine was added for the final 5 h of culture and incorporation of radioactivity was assessed by scintillation spectroscopy. Results are expressed as percent suppression (mean ± SE, n = 4) of DNA synthesis in Mv1Lu cells. Decorin and LAP reversed suppression in Mv1Lu cells by supernatants of PBMCs of TB patients.