Table 1.
Precoating | Competitor used | % reduction of binding of [125I]TGF-β
|
|
---|---|---|---|
Exp. 1 | Exp. 2 | ||
Decorin | Decorin | 78 | 63 |
BSA | 16 | 11 | |
LAP | LAP | 60 | 92 |
BSA | 13 | 11 |
Samples containing [125I]TGF-β alone or in combination with decorin (50 μg/ml), LAP (100 ng/ml), or BSA (50 μg/ml) were added to microtiter plates precoated with decorin or LAP. The bound radioactivity was solubilized and counted in a γ counter. Results represent percent reduction of binding of [125I]TGF-β in two experiments performed.