Table 2.

Production of IFN-γ and TGF-β by PBMCs of TB patients and control subjects cocultured with optimal concentrations of antibody to TGF-β, decorin, or LAP

Stimulus	TB patients		Controls
	IFN-γ	TGF-β	IFN-γ	TGF-β
NIL	<0.03	1.3  ±  0.5	<0.03	0.7  ±  0.3
PPD	0.224  ±  0.08	13.5  ±  3.6*	2.7  ±  0.6	5.3  ±  1.3
PPD + NAB	0.442  ±  0.12†	3.8  ±  1.3‡	2.6  ±  0.5	2.2  ±  1.3
PPD + DEC	0.556  ±  0.15†	4.1  ±  3.6§	2.5  ±  0.5	2.2  ±  1.2
PPD + LAP	0.803  ±  0.22¶	3.9  ±  2.3§	2.8  ±  0.5	1.7  ±  1.3

PBMCs of TB patients and healthy tuberculin-reactive control subjects were cultured for 72 h with or without PPD (10 μg/ml) in the presence or absence of neutralizing antibody to TGF-β (NAB) (10 μg/ml), decorin (DEC) (50 μg/ml), or LAP (100 ng/ml). IFN-γ and TGF-β immunoreactivities in culture supernatants were assessed by ELISA and both are expressed as ng/ml. Results represent mean ± SE, n = 8. 

^*P ≤ 0.0008 compared with TGF-β in supernatants of unstimulated PBMCs from TB patients. 

^†P ≤ 0.005 compared with IFN-γ in supernatants of PPD-stimulated PBMCs from TB patients. 

^‡P ≤ 0.006 compared with TGF-β in supernatants of PBMCs from TB patients cultured with PPD alone. 

^§P ≤ 0.004 compared with TGF-β in supernatants of PBMCs from TB patients cultured with PPD alone. 

^¶P ≤ 0.03 compared with IFN-γ in supernatants of PPD-stimulated PBMCs from TB patients.