Table 2.
Inflammatory response in native and transplanted hearts
| Group | n | I.S. | CD8 | CD4 | TNF-α | TGF-β | IFN-γ | IL-1 | IL-2 | IL-6 | MHC I | MHC II | ICAM-1 | VCAM-1 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Normal | ||||||||||||||
| Native | 6 | − | − | − | − | − | − | − | − | − | − | − | +/− | − |
| Transplanted | 6 | − | − | − | − | − | − | − | − | − | − | − | +/− | − |
| Acute infection | ||||||||||||||
| Native | 4 | ++ | ++ | + | + | + | − | − | − | − | ND | ND | ND | ND |
| Transplanted | 4 | ++ | ++ | + | +/++ | +/++ | − | +/− | ND | − | ND | ND | ND | ND |
| Chronic infection | ||||||||||||||
| Native | 19 | +++ | ++/+++ | + | ++ | ++ | − | +/++ | − | +/++ | ++/+++ | ++/ | ++/+++ | +/++ |
| +++ | ||||||||||||||
| Transplanted | 5 | − | − | − | − | − | − | − | − | − | + | + | + | + |
| Transplanted plus PBS | 4 | − | − | − | − | − | − | − | − | − | + | + | + | + |
| Transplanted plus parasites | 10 | ++/+++ | ++/+++ | + | ++ | ++ | − | +/++ | − | +/++ | + | +/++ | +/++ | + |
| Transplanted plus killed parasites | 3 | +/++ | + | + | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| Allotransplanted | 2 | +++ | ++ | ++/++ | ++ | ++ | + | ++ | + | + | ++ | ++ | ND | ++ |
Scores for the degree of inflammation (I.S.) and for cell surface markers and cytokine-producing cells were obtained as described. Transplanted hearts in normal or chronically infected (>200 days post-infection) mice were examined 20–60 days posttransplantation. Injections of PBS or heat-killed parasites (1 million in 10 μl) were made into hearts 2–14 weeks posttransplantation and examined 10–50 days later. Transplants into acutely infected mice were done at day 24 post-infection and examined 15 days later. Allografted hearts were examined 14 days posttransplantation. n, number of hearts examined; ND, not determined; TNF-α, tumor necrosis factor α; TGF-β, transforming growth factor-β; IFN-γ, interferon γ; IL, interleukin; ICAM-1, intercellular adhesion molecule 1; VCAM-1, vascular adhesion molecule 1.