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. 1997 Apr 15;94(8):3954–3959. doi: 10.1073/pnas.94.8.3954

Figure 4.

Figure 4

HIV-1 transactivation of the c-kit promoter. Transcriptional activation in 293 cells was determined by measuring CAT activity of the reporter construct pCD161 driven by the human c-kit promoter. 293 cells were transfected with 2 μg of CD161 and the indicated amounts (a–c) or 20 μg (d) of plasmid encoding full-length HIV-1 proviral DNA (NL4–3, SG3.1, YU2, or HXE) or HIV-1 Nef, Rev, Vpr, or Tat expression plasmids. Cell lysates prepared 48 h after transfection were assayed for CAT activity (20). All determinations were normalized to the activity of cotransfected pCMV-βgal. (a) pCD161 was cotransfected with different amounts of pNL4–3 HIV-1 proviral DNA. (b–d) Transactivation of the c-kit promoter by different HIV-1 isolates (b), HIV-1 proteins (c), or nef alleles (d). The Nef(Eli) plasmid was used in c. The relative activity above the basal level is shown at the top of each thin layer chromatography plate or bar graph. In d, the cpm were from converted acetylated chloramphenicol. Results shown are from single experiments that were repeated two or three times with similar results.