Figure 1.

Detection of HL-60 cells in peripheral blood of leukemic SCID mice by RT-PCR amplification of human c-myb transcripts. c-myb and β-actin transcripts were separately detected by RT-PCR using leukocyte total RNA from 0.1 ml of peripheral blood from leukemic mice (three mice per group), 21 or 35 days after injection of HL-60 cells and systemic treatment with MM or AS c-myc ODNs. Blots were hybridized with a γ-³²P-end-labeled c-myb or β-actin oligomer complementary to a region of the amplified 230-bp c-myb or 210-bp β-actin segment, respectively. Blots were exposed for 24 (c-myb) and 2 (β-actin) hr and are representative of two different experiments.