SSCP analysis of exon 7 from patients 9394, 9561, 9318, 9385, HDWT7, and HDWT6. Only a slight shift can be seen in the upper bands in patient 9318 under the conditions used for this gel. NEK control DNA is heterozygous for the RFLP (alleles A and B) and patient 9501 is homozygous for allele A. Patients HDWT7 and HDWT6 are homozygous for allele A and the additional bands correspond to the mutation, which is identical to the mutation in patient 9385. Lanes 9394, 9561, 9318, and 9385 contain tumor DNA; only altered bands are seen.