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BsiEI digests of exon 7 PCR products from blood (B) and tumor (T) DNA from patient 9318 with a bilateral WT and a normal control (NEK). Loss of the recognition sequence by the mutation results in an undigested fragment of 235 bp; the digested products are 80 and 155 bp. M, 1-kb size marker. Schematic shows the mutation with loss of BsiEI site and the stop codon.
