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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Reprod Toxicol. 2007 Jan 19;23(3):267–282. doi: 10.1016/j.reprotox.2007.01.004

Figure 4.

Figure 4

Diagram showing the procedures of restriction landmark genomic scanning (RLGS). Methylation detection in the RLGS profile depends on the methylation sensitivity of NotI. NotI, which recognizes CG-rich regions and cannot cleave DNA sequences when 5-cytosine is methylated, acts as the landmark in the profiles. Three types of methylation status are expected, depending on which allele is methylated. mNotI represents the methylated site of the enzymes. Following further endonuclease digestion of EcoRV (1D gel electrophoresis) and HinfI (2D gel electrophoresis), the RLGS profile will result in a change in spot intensity that quantitatively reflects the copy number and methylation status of the NotI fragment