Table 1.
Additive | Conc., μg/ml | CFU*
|
|||
---|---|---|---|---|---|
Total | G/GM/M | Mix | HPP-Mix | ||
None | — | 95.4 (7.93) | 50.1 (8.52) | 30.5 (2.68) | 13.2 (2.58) |
dlk | 0.1 | 95.9 (6.86) | 59.2 (9.31) | 23.9 (3.08) | 12.8 (1.82) |
0.5 | 82, 113 | 43, 73 | 26, 22 | 13, 18 | |
1.0 | 99, 110 | 50, 70 | 31, 22 | 18, 18 | |
Control | 0.1 | 95.5 (6.53) | 59.1 (9.31) | 24.5 (3.82) | 11.8 (1.42) |
0.5 | 78, 121 | 43, 82 | 17, 24 | 18, 14 | |
1.0 | 77, 104 | 41, 67 | 24, 21 | 11, 16 |
Four fetal liver purifications provided the stem cells for the studies with no additive, and 0.1 μg/ml and the data are expressed as the mean of these experiments, with SEM in parentheses. Data from two separate experiments with additive at 0.5 and 1.0 μg/ml are shown.
CFU are expressed per 1,000 AA4.1+, lin−/lo, Sca-1+ and c-kit+ fetal liver cells. G, granulocyte; M, macrophage; GM, granulocyte/macrophage; Mix, contain 2 or more myeloid lineage cells and erythroid bursts, HPP ≥ 1 mm in 8 days.