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Purification of GPI precursors and hydrolysis fragments by TLC. (A) 2D TLC of P. falciparum [3H]-glucosamine-labeled GPI precursors (22, 23). Asterisks indicate uncharacterized glycolipids. (B) TLC-purified GPI (Pfgl-α, Rf 0.2) was hydrolyzed enzymatically and the products resolved by 1D TLC in CMW 4:4:1 [GPI-PLD yielding 100% conversion to IPG, Rf 0.01; PLA2 yielding 35% conversion to lyso-GPI, as previously reported (22), Rf 0.1].
