Figure 5.

Activation of PKC by GPI-associated diacylglycerols. (A) Macrophages were stimulated with medium, PDBu, GPI, lyso-GPI, or IPG, and the cytosol-to-membrane translocation of nPKCɛ determined by Western blot analysis. (B) Activation of nPKCɛ by mfVSG-GPI, malarial GPI, and crude malaria parasite extracts (PE), but not the alkylacylglycerol-containing iM4 GIPL of L. mexicana. Other PKC isoforms in these cells were unaffected by all agonists except PDBu (not shown).