Figure 6.

(A) Activation of both PKC and PTK by GPI and parasite extracts is required for downstream gene expression. The PTK and PKC-specific antagonists herbimycin A (Herb.A) and calphostin C (Cal.C) were able at 0.1 μM and 1 μM, respectively, to inhibit substantially TNF output in response to GPI and whole parasite extracts (PE). (B) IPG cooperates with PDBu in downstream TNF expression. (C) TNF output in response to crude parasites extracts (PE) and GPI is inhibited by 5 μg/ml antiGPI monoclonal 1C7.