Figure 2.

(A) Time course of the disappearance of donor monocytes, purified from the blood of a wild-type donor (NAT-R) and injected intravenously into a mutant recipient (NAT-S). Monocytes were purified from ≈150 ml of donor blood and ≈1 × 10⁷ cells were injected. Results are expressed as a percentage of the value at 30 min. (B) Time course of the disappearance of donor monocytes from the blood of a mutant recipient rabbit (NAT-S) after intravenous injection of 45 ml of whole blood from a wild-type donor (NAT-R). Monocytes were recovered from timed blood samples by first isolating the mononuclear cell fraction by density gradient centrifugation and then allowing the monocytes to adhere to plastic plates previously coated with microexudate secreted by BHK cells. Results are expressed relative to the value at 23 min. Values for duplicate analyses at each time point are shown.