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. 1997 Apr 15;94(8):4050–4055. doi: 10.1073/pnas.94.8.4050

Figure 2.

Figure 2

Fluorescence and ImmunoGold (Janssen) localization of pVL1393–23GFP infected cells. (A and B) 23GFP autofluorescence in pVL1393-23GFP-infected Sf9 cells. (a and b) GFP autofluorescence and phase contract double exposure. (A and a) Forty-eight hours postinfection. (B and b) Seventy-two hours postinfection. Exposure time for cells at different time points postinfection was constant and set for exposure of the 72-hr postinfection. (C) ImmunoGold labeling (48 hr postinfection) using antiserum to GFP (1:1500; CLONTECH) and secondary gold conjugate. Fixation and ImmunoGold labeling of infected were done as described in Hong et al. (5). ( Created by potrace 1.16, written by Peter Selinger 2001-2019 ), Intranuclear microvesicles, (↔), cytoplasmic membranes condensed near the nuclear envelope. Protein was detected as in C. C, cytoplasm; n, nucleus.