Figure 3.

ImmunoGold localization of ODV-E25, 23β-gal, 23GFP, 24GFP, and ODV-E66. All data are presented from Sf9 infected cells at 48 hr postinfection and secondary antibody linked to gold. (A) 23β-gal recombinant virus (anti-β-gal); (B) AcMNPV (E2 strain; anti-ODV-E25 1:1000). (C) 23GFP recombinant virus (anti-GFP). (D) 24GFP recombinant virus (anti-GFP). (E) pVL-E66 recombinant virus (anti-ODV-E66, ref. 5). n, nucleus; C, cytoplasm; m, microvesicles; ➯, labeling of ODV envelope; ➤, labeling of the nuclear envelope; →, labeling of cytoplasmic membranes; ↔, condensation of cytoplasmic membranes to the periphery of the nucleus. (Bar = 1 μm.)