Figure 3.

Killing of HeLa cells by wild-type and recombinant H. ducreyi cdt gene products. Partial monolayers of HeLa cells (20–30% confluent) in 24-well tissue culture plates were exposed to culture supernatants (A–D) or to bacterial culture medium (E) for 2 hr. The wells were stained and photographed 72 hr later. (A) H. ducreyi strain 35000; (B) H. ducreyi strain 512; (C) E. coli DH5α(pLDC103); (D) E. coli DH5α(pCRII); (E) Luria–Bertani culture medium. (Bar = 50 μm.)