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. 1997 Apr 15;94(8):4056–4061. doi: 10.1073/pnas.94.8.4056

Figure 4.

Figure 4

Fluorographic detection of H. ducreyi CDT proteins expressed in vitro. The H. ducreyi cdtA gene (lane C), the H. ducreyi cdtB gene (lane E), and the H. ducreyi cdtC gene (lane G) were each amplified from the chromosome of H. ducreyi strain 35000 and used in an in vitro coupled transcription/translation system for linear DNA templates to establish the size of the protein product of each gene. The position of CdtA is indicated by ∗, that of CdtB by ▪, and that of CdtC by ○. The vector pCRII (lane A) and recombinant plasmids containing the H. ducreyi cdtA gene (pLDC101, lane B), the H. ducreyi cdtAB gene pair (pLDC102, lane D), and the entire H. ducreyi cdtABC gene cluster (pLDC103, lane F) were used in an in vitro coupled transcription/translation system for circular DNA templates. The HeLa cell killing ability (cytotoxic activity) of culture supernatants from E. coli DH5α-derived recombinant strains containing pLDC101, pLDC102, pLDC103, and the vector plasmid pCRII is indicated. ND, not determined.