Figure 1.

(A) Growth kinetics of SIVagm3Δnef and of SIVagm3 (nef open) in MOLT-4/8 cells. MOLT-4/8 cells were infected in duplicate at a multiplicity of infection of 4 × 10⁻⁴ either with SIVagm3Δnef, SIVagm3 (nef open), or exposed to medium alone. Supernatants were assayed for RT activity. (B) Growth kinetics of SIVagm3Δnef and of SIVagm3 on phytohemagglutinin blasts of an SIVagm-negative African green monkey or a pig-tailed macaque. PBMCs were infected with a multiplicity of infection of 3 × 10⁻⁴ either with SIVagm3Δnef, SIVagm3 (nef open), or exposed to medium alone. Supernatants were assayed for RT activity. M. nem, Macaca nemestrina.