Table 1.
Virus reisolation and diagnostic PCR
| wpi* | Cell no. used for
|
AGM 111 | AGM 112 | AGM 113 | AGM 115 | AGM 158 | AGM 159 | AGM 160 | AGM 190 | |
|---|---|---|---|---|---|---|---|---|---|---|
| Reisolation | PCR | |||||||||
| 0 | 1 × 106 PBMC | 2 × 105 PBMC | −(−) | −(−) | −(−) | −(−) | ||||
| 1 | 1 × 106 PBMC | 2 × 105 PBMC | −(−) | −(−) | −(−) | −(−) | ||||
| 2 | 1 × 106 PBMC | 2 × 105 PBMC | −(−) | −(−) | −(−) | −(−) | ||||
| 3 | 1 × 106 PBMC | 2 × 105 PBMC | −(−) | −(−) | +(Δnef) | −(−) | ||||
| 4 | 3 × 106 PBMC | 2 × 105 PBMC | −(−) | −(−) | −(−) | −(−) | ||||
| 6 | 1–2 × 106 CD4+ PBMC | ND | −(ND) | −(ND) | −(ND) | −(ND) | ||||
| 8 | 5–10 × 106 CD4+ PBMC | 2 × 105 CD4+ PBMC | −(−) | −(−) | −(−) | −(−) | ||||
| 11 | 1–2 × 106 CD4+ PBMC | 2 × 105 CD4+ PBMC | −(−) | −(−) | −(−) | −(−) | ||||
| 16 | 1 × 107 PBMC | 2 × 105 PBMC | −(−) | −(−) | −(−) | −(−) | ||||
| 21 | 0.7–1.7 × 107 PBMC | 2 × 105 PBMC | −(Δnef) | −(−) | −(−) | −(−) | ||||
| 5–7 × 106 LNMC | 2 × 105 LNMC | −(ND) | −(Δnef) | −(Δnef) | −(−) | |||||
| 25 | 0.5–1 × 107 PBMC | 2 × 105 PBMC | −(−) | −(−) | −(−) | −(−) | ||||
| 31 | 1–2 × 106 PBMC | 2 × 105 PBMC | −(Δnef) | −(−) | −(−) | −(−) | −(−) | −(−) | −(−) | −(−) |
| 32 | 1 × 106 PBMC | 2 × 105 PBMC | −(Δnef) | −(−) | −(−) | −(−) | −(−) | −(−) | −(−) | −(−) |
| 33 | 1 × 106 PBMC | 2 × 105 PBMC | −(−) | −(−) | +(−) | −(−) | +(wt) | +(−) | +(wt) | −(−) |
| 34 | 1 × 106 PBMC | 2 × 105 PBMC | −(−) | +(wt) | +(−) | −(−) | +(wt) | +(wt) | +(wt) | +(−) |
| 35 | 1 × 106 PBMC | 2 × 105 PBMC | −(−) | +(−) | +(−) | −(−) | +(wt) | +(wt) | +(wt) | +(−) |
| 37 | 1 × 106 PBMC | 2 × 105 PBMC | −(−) | +(−) | −(−) | −(−) | +(wt) | +(wt) | +(wt) | +(−) |
| 39 | 1 × 106 PBMC | 2 × 105 PBMC | +(−) | +(−) | −(−) | −(−) | +(wt) | +(wt) | −(wt) | −(−) |
| 43 | 1 × 106 PBMC | 2 × 105 PBMC | −(−) | +(−) | −(−) | −(−) | +(wt) | +(wt) | +(wt) | −(−) |
| 47 | 1 × 106 PBMC | 2 × 105 PBMC | +(wt) | +(−) | +(−) | −(wt) | +(wt) | −(wt) | +(wt) | −(−) |
| 51 | 1 × 106 PBMC | 2 × 105 PBMC | +(wt) | +(−) | +(−) | −(wt) | +(wt) | +(wt) | +(wt) | −(−) |
| 55 | 1 × 106 PBMC | 2 ×105 PBMC | +(wt) | +(−) | −(−) | −(wt) | +(wt) | ND (wt) | +(wt) | −(wt) |
Results of diagnostic PCR in brackets. Δnef, Δnef proviral sequence; wt, wild-type proviral sequence; ND, not determined.
*
wpi, weeks post immunization.