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Effect of CCCP and DNP. Cells were treated by Method A followed by an incubation of 80 min. CCCP and DNP were added at the same time as thioredoxin. Tetracycline (20 μg/ml) was added to prevent residual protein synthesis. •, A307 (unc+), CCCP; ○, K1640 (Δunc), CCCP; ▴, A307 (unc+), DNP; ▵, K1640 (Δunc), DNP.
