Table 1.

Comparison of cell disruption methods

Method	Treatment*	cfu†	Relative yield:‡ TrxA at 10 min vs. TrxA at 0 min
A	Sucrose-EDTA-lysozyme	1.3 ± 0.7 × 105	0.2
B	Sucrose-EDTA; freeze–thaw	2.2 ± 1.3 × 107	10−4
C	Freeze–thaw	1.7 ± 1.6 × 108	10−3
	No treatment	<102	ND

TrxA, thioredoxin; ND, not done. 

^*Details are given in Materials and Methods. 

^†Average of four to five experiments. 

^‡Samples were either resuspended in thioredoxin-containing buffer (0 min) or resuspended without thioredoxin and kept on ice for 10 min before thioredoxin addition. The samples were then incubated at 25°C for 100 min.