Table 2.

Requirements for particle assembly

Experiment	Defective component	Relative yield
a	Thioredoxin (G92D)	0.0000
b	pIV	0.0001
c	PS	0.003
d	PS (pI suppressor of ΔPS)	0.25

Experiments: a, wild-type thioredoxin vs. mutant thioredoxin containing a Gly-92 → Asp substitution; b, R172 (standard helper phage) vs. R184 (gene IV^amber helper phage) and pMac5-8 (standard phagemid); c, pPMR30 (wild type) vs. its packaging signal deletion derivative, pPMR30Δ17 (ΔPS). These phagemids confer Amp^R; therefore, transducing particles were scored as Amp^R Tet^R colonies. d, helper phage R472, containing a gene I suppressor of the ΔPS mutation and phagemid pPMR30 vs. pPMR30Δ17. Experiments were by Method B with incubations of 130 min, except for b, which was by Method C with an incubation of 100 min.