Skip to main content
. 1997 Apr 15;94(8):4068–4073. doi: 10.1073/pnas.94.8.4068

Table 3.

Reversal of CCCP inhibition by nucleotides

Additions Relative yield, %
unc+ Δunc
None 100 100
1 μM CCCP 0.2 0.3
1 μM CCCP + 10 mM ATP 0.5 40
3 μM CCCP + 10 mM ATP 0.4 30
10 μM CCCP + 10 mM ATP 0.04 12
30 μM CCCP + 10 mM ATP <0.02 5
30 μM CCCP + 30 mM ATP* ND 1.3
30 μM CCCP + 10 mM AMP/GMP-PMP <0.02 <0.04

Experimental conditions were as in Fig. 6. ND, not done. 

*

From a separate experiment in which Mg2+ was 40 mM to compensate for the higher ATP. The control experiment (40 mM Mg2+ + 30 mM ATP without CCCP) had similar value as the one done at the standard condition (20 mM Mg2+ + 10 mM ATP).