Skip to main content
. 1997 Apr 15;94(8):4074–4079. doi: 10.1073/pnas.94.8.4074

Figure 4.

Figure 4

In vitro packaging of labeled plus strands showing that segment L can be packaged in the absence of M if segment S is made larger. The RNA in the packaging mixtures is shown below each lane. Segment S is derived from pLM658, segment M from 656, and segment L from pLM1157 as in Fig. 2. Segment S7 is the transcript of pLM1725 (Fig. 1) which is 6.8 kb. Note that segment S packages alone, whereas M and L package poorly alone. Segment M packages well in the presence of S and segment L packages well in the presence of S and M. Note that segment S7 packages alone (lane 4) and that segment L packages with segment S7 (lane 8) but not well with either S alone or M alone.