Figure 1.
Detection of donor cells in the brain after bone marrow transplantation with retrovirally tagged bone marrow cells. Arrows indicate representative cells positive by ISHH with 35S-labeled oligonucleotide (A–C) or riboprobe (D). (A and B) Bright (A) and dark (B) field photographs of the same section. ISHH-positive cells (arrows) detected in the hippocampus of an animal 14 days postbone marrow transplantation. (C) Positive cells in the region of the septum of an animal sacrificed 14 days after bone marrow transplantation. The photograph is a double exposure of a bright field image with a dark field image of the same area. The dark field image was photographed using a red filter so that the autoradiographic grains would appear red. (D) A cell (arrow) within the ependyma of the third ventricle. [Bars = 10 μm (A–C) and 40 μm (D).]