Table 1.

DHR fluorescence in GT1-1 trk cells

Condition	10 min	40 min
+ NGF	8 ± 4	44 ± 5
− NGF	57 ± 5*	181 ± 9*
+ 100 μM Meclofenamate	30 ± 2	125 ± 6†
+ 20 μM Rotenone	22 ± 6†	94 ± 14†
NGF + 10 μM PD98059	19 ± 4	53 ± 4
NGF + 50 μM PD98059	54 ± 6*	152 ± 7*
NGF + 100 μM PD98059	68 ± 5*	179 ± 8*
No calcium
+ NGF	35 ± 4	86 ± 8†
− NGF	30 ± 5	84 ± 7†

Free radical production in GT1-1 trk cells detected as increased fluorescence due to oxidation of DHR to rhodamine 123. Values represent the percent change in brightness from basal values obtained just after loading DHR 123. All drugs were added as a 30-min pretreatment by including the drug with the DHR loading solution. Solutions used for most experiments contained 1.8 mM calcium, whereas solutions for the low-calcium condition contained no added calcium but did not include a calcium chelator to drop the calcium concentration to 0. Values represent the mean ± SEM for n = 40–300 for each group. 

^*Versus +NGF condition. P < 0.05 by ANOVA followed by Student–Newman–Keuls. 

^†Versus −NGF condition. P < 0.05 by ANOVA followed by Student–Newman–Keuls.