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Oscillations of Per protein in the nuclei of prothoracic gland cells. Pupae reared at 25°C in LD were sectioned and fixed every 6 hr. After staining with anti-Per and DAPI, the intensity of Per immunofluorescence in the nuclei of prothoracic gland cells was quantitated as described. Each point represents the mean intensity/pixel (± SEM) of Per immunofluorescence in prothoracic gland nuclei of one to three animals, based on measurements from at least two sections.
