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Immunoblots of sarkosyl-insoluble tau from familial MSTD brain before (lane 3) and after (lane 2) alkaline phosphatase treatment using antiserum BR133. Note that before alkaline phosphatase treatment, two major tau bands of 64 and 68 kDa and a minor band of 72 kDa are present; after alkaline phosphatase treatment, two major bands are visible that align with recombinant tau isoforms (lane 1) of 383 and 412 amino acids (isoforms D and E in schematic diagram).
