Table 1.

Purification of IPF from calf brain cytosol

Fraction	Total protein, mg	IC50, mg/0.12 ml	Total activity, units	Yield, %	Specific activity, units/mg	Purification, -fold
Cytosol	69,960	0.50	139,920	100	2	1
45% AS ppt	26,920	0.25	107,680	77	4	2
0.5 M PAE	6,952	0.081	85,827	61	12	6
0.05 M HTP	1,824	0.040	45,600	33	25	13
0.3 M Yellow-86	58	0.011	5,273	3.8	91	46
Superdex peak	23	0.0050	4,600	3.3	200	100
Sucrose peak	2.5	0.0011	2,273	1.6	909	455
Mono Q IPF α	0.25	0.00043	581	0.42	2,326	1,160
Mono Q IPF β	0.25	0.00039	641	0.46	2,564	1,280