Figure 3.

Autoradiographic binding of a GABA site ligand ([³H]muscimol) and a benzodiazepine site ligand ([³H]Ro15-4513) to representative horizontal brain sections from newborn wild-type (+/+), heterozygous (+/−), and homozygous (−/−) mice. (A) Total binding of 5 nM [³H]Ro15-4513. (B) Binding of [³H]Ro15-4513 in the presence of 30 μM zolpidem. (C) Total binding of 20 nM [³H]muscimol. All images represent specific binding since [³H]Ro15-4513 and [³H]muscimol failed to label any sites in the presence of 10 μM flumazenil and 100 μM GABA, respectively.