Table 1.
Binding of [3H]muscimol and [3H]Ro15-4513 to whole brain homogenates prepared from postnatal day 1 (pups) or 8- to 12-week-old (adult) mice
| Binding parameter | Wild type (β3+/+) | Homozygous (β3−/−) | P |
|---|---|---|---|
| [3H]Muscimol (pups) | |||
| Bmax, pmol/mg | 3.0 ± 0.2 | 1.5 ± 0.2 | <0.001 |
| Kd, nM | 10.8 ± 1.1 | 9.1 ± 3.3 | 0.62 |
| [3H]Ro15-4513 (pups) | |||
| Bmax, pmol/mg | 1.5 ± 0.1 | 0.7 ± 0.1 | <0.001 |
| Kd, nM | 8.0 ± 2.9 | 8.8 ± 3.4 | 0.86 |
| [3H]Ro15-4513, adult | |||
| Bmax, pmol/mg | 3.1 ± 0.3 | 1.7 ± 0.1 | 0.01 |
| Zolpidem + [3H]Ro15-4513 (pups) IC50, μM | 0.54 ± 0.09 | 0.15 ± 0.04 | <0.001 |