Table 2.
Binding of [3H]muscimol and [3H]Ro15-4513 to postnatal day 1 mouse brain sections as revealed by autoradiography
| Brain region | [3H]Muscimol, nCi/mg | [3H]Ro15-4513, nCi/mg |
|---|---|---|
| Frontoparietal cortex | ||
| β3+/+ | 0.80 ± 0.14 | 3.70 ± 0.84 |
| β3+/− | 0.72 ± 0.10 | 2.58 ± 0.33 |
| β3−/− | 0.58 ± 0.09* | 2.59 ± 0.52* |
| Olfactory bulbs | ||
| β3+/+ | 0.65 ± 0.08 | 2.43 ± 0.40 |
| β3+/− | 0.55 ± 0.07 | 2.16 ± 0.46 |
| β3−/− | 0.59 ± 0.05 | 1.00 ± 0.08† |
| Caudate/putamen | ||
| β3+/+ | 0.66 ± 0.07 | 3.42 ± 0.63 |
| β3+/− | 0.64 ± 0.14 | 3.15 ± 0.34 |
| β3−/− | 0.53 ± 0.10 | 2.57 ± 0.37* |
| Thalamus | ||
| β3+/+ | 0.83 ± 0.16 | 4.92 ± 0.62 |
| β3+/− | 0.81 ± 0.22 | 3.79 ± 0.40 |
| β3−/− | 0.52 ± 0.06* | 2.51 ± 0.85† |
| Hippocampus | ||
| β3+/+ | 0.70 ± 0.11 | 3.68 ± 0.90 |
| β3+/− | 0.64 ± 0.14 | 2.96 ± 0.80 |
| β3−/− | 0.57 ± 0.16 | 2.22 ± 0.48* |
Optical density values in relation to radioactivity standards are means ± SEM for three to six brains. THIP (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol) (10 μM) was able to reduce [3H]muscimol binding to the background level (data not shown). The statistical significance of differences between β3+/+, β3+/−, and β3−/− mice was determined using one-way ANOVA followed by Bonferroni post test (∗, P < 0.05;
†
, P < 0.001).