Figure 2.

EAAT5 mRNA is most abundantly expressed in the retina. The EAAT5 cDNA was used for hybridization of Northern-blotted poly(A)⁺ RNAs (2 μg) from retina and other human tissues. The retinal signal, a single 3.1-kb band, was overexposed here so that additional EAAT5-hybridizing bands in other tissue RNAs could be seen. Lower, the same blots were hybridized with a β-actin probe to control for RNA loading in each lane.