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. 1992 Apr;174(7):2230–2235. doi: 10.1128/jb.174.7.2230-2235.1992

Chemical characterization of pH-dependent structural epitopes of lipopolysaccharides from Rhizobium leguminosarum biovar phaseoli.

U R Bhat 1, R W Carlson 1
PMCID: PMC205843  PMID: 1372601

Abstract

Lipopolysaccharide (LPS) was isolated from free-living Rhizobium leguminosarum bv. phaseoli CE3 cells grown at pH 4.8 (antigenically similar to bacteroid LPS) and compared with that from cells grown at pH 7.2 (free-living bacteria). Composition analysis revealed that pH 7.2 LPS differs from pH 4.8 LPS in that 2,3,4-tri-O-methylfucose is replaced by 2,3-di-O-methylfucose. The amount of 2-O-methylrhamnose is greater in the pH 4.8 LPS than in the pH 7.2 LPS. Analysis of the structural components of LPS (O-chain polysaccharide, core oligosaccharides, and the lipid A) revealed that all the composition differences in the various LPSs occur in the O-chain polysaccharide. These structural variations between pH 4.8 and pH 7.2 LPSs provide a chemical basis for the observed lack of cross-reactivity with pH 4.8 LPS of two monoclonal antibodies, JIM28 and JIM29, raised against free-living bacteria grown at pH 7.2. An LPS preparation isolated from bacteroids contained both 2,3,4-tri-O- and 2,3-di-O-methylfucose residues. This result is consistent with the finding that the two monoclonal antibodies react weakly with bacteroid LPS. It is concluded that methylation changes occur on the LPS O-chain of R. leguminosarum bv. phaseoli when the bacteria are grown at low pH and during nodule development.

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Selected References

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  1. Bhat U. R., Carlson R. W., Busch M., Mayer H. Distribution and phylogenetic significance of 27-hydroxy-octacosanoic acid in lipopolysaccharides from bacteria belonging to the alpha-2 subgroup of Proteobacteria. Int J Syst Bacteriol. 1991 Apr;41(2):213–217. doi: 10.1099/00207713-41-2-213. [DOI] [PubMed] [Google Scholar]
  2. Bhat U. R., Krishnaiah B. S., Carlson R. W. Re-examination of the structures of the lipopolysaccharide core oligosaccharides from Rhizobium leguminosarum biovar phaseoli. Carbohydr Res. 1991 Nov 11;220:219–227. doi: 10.1016/0008-6215(91)80020-n. [DOI] [PubMed] [Google Scholar]
  3. Bhat U. R., Mayer H., Yokota A., Hollingsworth R. I., Carlson R. W. Occurrence of lipid A variants with 27-hydroxyoctacosanoic acid in lipopolysaccharides from members of the family Rhizobiaceae. J Bacteriol. 1991 Apr;173(7):2155–2159. doi: 10.1128/jb.173.7.2155-2159.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973 Aug;54(2):484–489. doi: 10.1016/0003-2697(73)90377-1. [DOI] [PubMed] [Google Scholar]
  5. Brink B. A., Miller J., Carlson R. W., Noel K. D. Expression of Rhizobium leguminosarum CFN42 genes for lipopolysaccharide in strains derived from different R. leguminosarum soil isolates. J Bacteriol. 1990 Feb;172(2):548–555. doi: 10.1128/jb.172.2.548-555.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Caetano Anollés G., Favelukes G. Host-Symbiont Specificity Expressed during Early Adsorption of Rhizobium meliloti to the Root Surface of Alfalfa. Appl Environ Microbiol. 1986 Aug;52(2):377–382. doi: 10.1128/aem.52.2.377-382.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carlson R. W., Garci F., Noel D., Hollingsworth R. The structures of the lipopolysaccharide core components from Rhizobium leguminosarum biovar phaseoli CE3 and two of its symbiotic mutants, CE109 and CE309. Carbohydr Res. 1989 Dec 21;195(1):101–110. doi: 10.1016/0008-6215(89)85092-x. [DOI] [PubMed] [Google Scholar]
  8. Carlson R. W. Heterogeneity of Rhizobium lipopolysaccharides. J Bacteriol. 1984 Jun;158(3):1012–1017. doi: 10.1128/jb.158.3.1012-1017.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Carlson R. W., Kalembasa S., Turowski D., Pachori P., Noel K. D. Characterization of the lipopolysaccharide from a Rhizobium phaseoli mutant that is defective in infection thread development. J Bacteriol. 1987 Nov;169(11):4923–4928. doi: 10.1128/jb.169.11.4923-4928.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Carlson R. W., Sanders R. E., Napoli C., Albersheim P. Host-Symbiont Interactions: III. Purification and Partial Characterization of Rhizobium Lipopolysaccharides. Plant Physiol. 1978 Dec;62(6):912–917. doi: 10.1104/pp.62.6.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Carlson R. W., Shatters R., Duh J. L., Turnbull E., Hanley B., Rolfe B. G., Djordjevic M. A. The Isolation and Partial Characterization of the Lipopolysaccharides from Several Rhizobium trifolii Mutants Affected in Root Hair Infection. Plant Physiol. 1987 Jun;84(2):421–427. doi: 10.1104/pp.84.2.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Carlson R. W., Yadav M. Isolation and partial characterization of the extracellular polysaccharides and lipopolysaccharides from fast-growing Rhizobium japonicum USDA 205 and its Nod- mutant, HC205, which lacks the symbiotic plasmid. Appl Environ Microbiol. 1985 Nov;50(5):1219–1224. doi: 10.1128/aem.50.5.1219-1224.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Carrion M., Bhat U. R., Reuhs B., Carlson R. W. Isolation and characterization of the lipopolysaccharides from Bradyrhizobium japonicum. J Bacteriol. 1990 Apr;172(4):1725–1731. doi: 10.1128/jb.172.4.1725-1731.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Goosen-de Roo L., de Maagd R. A., Lugtenberg B. J. Antigenic changes in lipopolysaccharide I of Rhizobium leguminosarum bv. viciae in root nodules of Vicia sativa subsp. nigra occur during release from infection threads. J Bacteriol. 1991 May;173(10):3177–3183. doi: 10.1128/jb.173.10.3177-3183.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gray J. X., Rolfe B. G. Exopolysaccharide production in Rhizobium and its role in invasion. Mol Microbiol. 1990 Sep;4(9):1425–1431. doi: 10.1111/j.1365-2958.1990.tb02052.x. [DOI] [PubMed] [Google Scholar]
  16. Halverson L. J., Stacey G. Signal exchange in plant-microbe interactions. Microbiol Rev. 1986 Jun;50(2):193–225. doi: 10.1128/mr.50.2.193-225.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hollingsworth R. I., Carlson R. W. 27-Hydroxyoctacosanoic acid is a major structural fatty acyl component of the lipopolysaccharide of Rhizobium trifolii ANU 843. J Biol Chem. 1989 Jun 5;264(16):9300–9303. [PubMed] [Google Scholar]
  19. Hollingsworth R. I., Carlson R. W., Garcia F., Gage D. A. A new core tetrasaccharide component from the lipopolysaccharide of Rhizobium trifolii ANU 843. J Biol Chem. 1989 Jun 5;264(16):9294–9299. [PubMed] [Google Scholar]
  20. Kannenberg E. L., Brewin N. J. Expression of a cell surface antigen from Rhizobium leguminosarum 3841 is regulated by oxygen and pH. J Bacteriol. 1989 Sep;171(9):4543–4548. doi: 10.1128/jb.171.9.4543-4548.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mayberry W. R. Hydroxy fatty acids in Bacteroides species: D-(--)-3-hydroxy-15-methylhexadecanoate and its homologs. J Bacteriol. 1980 Aug;143(2):582–587. doi: 10.1128/jb.143.2.582-587.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Parniske M., Ahlborn B., Werner D. Isoflavonoid-inducible resistance to the phytoalexin glyceollin in soybean rhizobia. J Bacteriol. 1991 Jun;173(11):3432–3439. doi: 10.1128/jb.173.11.3432-3439.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Priefer U. B. Genes involved in lipopolysaccharide production and symbiosis are clustered on the chromosome of Rhizobium leguminosarum biovar viciae VF39. J Bacteriol. 1989 Nov;171(11):6161–6168. doi: 10.1128/jb.171.11.6161-6168.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Puvanesarajah V., Schell F. M., Gerhold D., Stacey G. Cell surface polysaccharides from Bradyrhizobium japonicum and a nonnodulating mutant. J Bacteriol. 1987 Jan;169(1):137–141. doi: 10.1128/jb.169.1.137-141.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sindhu S. S., Brewin N. J., Kannenberg E. L. Immunochemical analysis of lipopolysaccharides from free-living and endosymbiotic forms of Rhizobium leguminosarum. J Bacteriol. 1990 Apr;172(4):1804–1813. doi: 10.1128/jb.172.4.1804-1813.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sonesson A., Jantzen E., Bryn K., Larsson L., Eng J. Chemical composition of a lipopolysaccharide from Legionella pneumophila. Arch Microbiol. 1989;153(1):72–78. doi: 10.1007/BF00277544. [DOI] [PubMed] [Google Scholar]
  27. Stacey G., So J. S., Roth L. E., Lakshmi SK B., Carlson R. W. A lipopolysaccharide mutant of Bradyrhizobium japonicum that uncouples plant from bacterial differentiation. Mol Plant Microbe Interact. 1991 Jul-Aug;4(4):332–340. doi: 10.1094/mpmi-4-332. [DOI] [PubMed] [Google Scholar]
  28. Tao H., Brewin N. J., Noel K. D. Rhizobium leguminosarum CFN42 lipopolysaccharide antigenic changes induced by environmental conditions. J Bacteriol. 1992 Apr;174(7):2222–2229. doi: 10.1128/jb.174.7.2222-2229.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. VandenBosch K. A., Brewin N. J., Kannenberg E. L. Developmental regulation of a Rhizobium cell surface antigen during growth of pea root nodules. J Bacteriol. 1989 Sep;171(9):4537–4542. doi: 10.1128/jb.171.9.4537-4542.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wood E. A., Butcher G. W., Brewin N. J., Kannenberg E. L. Genetic derepression of a developmentally regulated lipopolysaccharide antigen from Rhizobium leguminosarum 3841. J Bacteriol. 1989 Sep;171(9):4549–4555. doi: 10.1128/jb.171.9.4549-4555.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. de Maagd R. A., Rao A. S., Mulders I. H., Goosen-de Roo L., van Loosdrecht M. C., Wijffelman C. A., Lugtenberg B. J. Isolation and characterization of mutants of Rhizobium leguminosarum bv. viciae 248 with altered lipopolysaccharides: possible role of surface charge or hydrophobicity in bacterial release from the infection thread. J Bacteriol. 1989 Feb;171(2):1143–1150. doi: 10.1128/jb.171.2.1143-1150.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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