Figure 2.

Inhibition of I_Ca,L by EPA in cultured neonatal rat ventricular myocytes. (A) Time course of the inhibitory effect of EPA on the peak I_Ca,L. The dotted short lines marked with 0.1, 5, and 40 represent the periods of extracellular puffing 0.1, 5, and 40 μM EPA. C, control; EPA, application of EPA; W, washout of EPA with the bath solution alone. (B) The concentration-dependent curve of EPA suppression of I_Ca,L. The number in parentheses represents individual ventricular myocytes treated with various concentrations of EPA. The IC₅₀ of EPA is 0.8 μM.