Table 2.

Effects of anandamide on blastocyst hatching in vitro

Treatment	No. of embryos cultured	No. of embryos developed to blastocysts	Hatched blastocysts
			No.	%
Anandamide, nM
0	42	41	24	58.5
10	42	41	22	53.7
20	45	43	14	32.6*
Anandamide	43	43	29	67.4
(20 nM) plus SR  (10 nM)
SR, 10 nM	31	31	21	67.7

Eight-cell embryos (10–12 embryos per group) were cultured in the presence or absence of anandamide and/or SR141716A (SR) for 84 h. Each experiment was repeated 3–4 times. 

*P < 0.05 (χ² test) compared with other groups.