Table 3.
Levels of NAPEs in the periimplantation mouse uterus
| Day of pregnancy | No. of determinations | Chain length:number of double bonds, pmol/μmol lipid phosphorus, mean ± SD
|
|||||
|---|---|---|---|---|---|---|---|
| 16:0 | 18:0 | 18:1n-9 | 18:1n-7 | 18:2n-6 | 20:4n-6 Anandamide* | ||
| 1 | 2 | 21.4 ± 0.4 | 10.4 ± 0.3 | 6.9 ± 0.4 | 4.9 ± 0.9 | 2.2 ± 1.6 | 1.5 ± 0.3 |
| 2 | 2 | 19.0 ± 0.6 | 11.2 ± 0.3 | 7.9 ± 0.3 | 3.9 ± 0.6 | 2.0 ± 1.4 | 1.7 ± 1.2 |
| 3 | 2 | 19.3 ± 6.0 | 17.8 ± 2.6 | 10.8 ± 2.0 | 4.9 ± 0.6 | 1.8 ± 1.2 | 2.1 ± 0.4 |
| 4 | 3 | 21.3 ± 0.3 | 15.0 ± 2.2 | 17.0 ± 5.6 | 4.3 ± 1.0 | 4.1 ± 1.7 | 2.0 ± 0.3 |
| 4, pseudopregnant | 2 | 22.0 ± 2.2 | 11.6 ± 0.9 | 6.5 ± 0.9 | 4.5 ± 0.2 | 2.6 ± 1.8 | 1.6 ± 0.03 |
| 5, pseudopregnant | 3 | 22.0 ± 3.7 | 14.0 ± 3.5 | 10.1 ± 2.7 | 4.0 ± 2.7 | 4.3 ± 2.0 | 1.9 ± 0.2 |
| 5, implant sites | 3 | 49.4 ± 3.0 | 29.2 ± 2.6 | 14.3 ± 2.1 | 6.5 ± 1.8 | 11.6 ± 1.9 | 5.1 ± 1.0 |
| 5, interimplant sites | 3 | 22.3 ± 3.4 | 17.3 ± 1.6 | 12.8 ± 3.5 | 6.9 ± 0.4 | 7.2 ± 1.1 | 2.7 ± 0.7 |
| 7, implant sites | 3 | 94.0 ± 22.6 | 117.2 ± 29.1 | 17.4 ± 2.0 | 17.3 ± 3.7 | 11.4 ± 5.7 | 5.2 ± 0.6 |
| 7, interimplant sites | 3 | 47.9 ± 1.5 | 34.0 ± 11.6 | 20.9 ± 1.9 | 6.0 ± 0.9 | 4.2 ± 2.1 | 11.9 ± 2.4 |
NAPEs isolated by TLC from lipid extracts of whole uteri, implantation sites, or interimplantation sites on indicated days of pseudopregnancy or pregnancy were reacted with phospholipase A2 and phospholipase D to generate NAEs. Levels of N-20:4 PE in interimplantation sites were significantly higher (P < 0.05) than those from implantation sites on day 7 of pregnancy (paired t-test).
*
Other polyunsaturated NAEs may also be present but were not included in this assay.